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The studied analyte was successfully extracted and determined with high efficiency using cloud point extraction method in water and biological matrices.
Like H2O2, nitrite ion ( NO 2 - ) is another often studied analyte in various fields including clinic, food, and environmental analyses because its excess level in the blood has been proved to lead to haemoglobin oxidation [23 26].
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The use of a biphenyl bonded column allowed to obtain a satisfactory selectivity for all the studied analytes.
For all studied analytes, the enantiomeric resolution was shown to be significantly influenced by the CD concentration.
To obtain satisfactory extraction efficiencies for the studied analytes, several parameters affecting the SALLE procedure were optimized.
The peak absorbance of all studied analytes was independent of geometry, suggesting that the separation of atomization and removal was attained.
The optimised method showed low method detection limits (3 35 ng L−1), acceptable accuracy (78 114%) and precision values (<13%) for most of the studied analytes regardless of the aqueous matrix.
The optimum values of desorption temperature were in the range 265 273° C, and desorption time were in the range 3.4 3.8 min. The limits of detection (LODs) and limits of quantitation (LO Qs of the studied analytes were found over the range of 0.03 0.04 ng/mL, and 0.1 0.13 ng/mL, respectively.
Fig. 1 Chemical structures of the studied analytes.
The effect of variation of n-Butanol concentration on the chromatographic behavior of the studied analytes was investigated in the concentration range of 5.0 12.0%.
The influence of different concentrations of SDS (0.05 0.175 M) on the selectivity, resolution and retention times of the studied analytes was investigated.
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