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Because adding a cluster from K = 5-6 did not provide much more information regarding the genetic structuring, assignment results were interpreted for K = 2-5.
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DT of protein structure has also been used as a generic computational tool to analyze various aspects of protein structure such as secondary structure assignment [ 20], structural classification [ 21, 22], and analysis of small-world nature of protein contacts [ 23].
Secondary structure assignment was possible for the assigned residues as shown in Figure 5A where chemical shift indices for the Cα carbons in GIG47 and its homolog 1TOG [ 21] are displayed.
Also, PPII helices are not assigned by the widely used Secondary Structure Assignment Method (SSAM): DSSP [21].
The purpose of this work is to propose simple rules to assign PPII based on a classical secondary structures assignment carried out using DSSP.
The secondary structure assignment of the sequences was carried out using STRuctural Alignments of Proteins (STRAP) (Gille and Frommel 2001).
Two STRUCTURE assignment and ancestry analyses were performed, one with prior population information assigned and the second with putative hybrid population identity set as unknown to examine alternate parentage contributions.
Therefore, any information derived from each technique used has to be reported to provide a comprehensive and meaningful overview on the structure assignment, since each technique will provide additional information consolidating the structural assignment or illustrating exclusion of alternative structures.
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