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The core of the package provides several variants of the RNA folding recursion: energy minimization, partition function and base pairing probabilities, backtracing of suboptimal structures, alignment-based as well as scanning versions.

Based on Dali Structures alignments no significant discrepancy was seen between the template and query 2D and 3D structures.

The regions in the sequence alignment do not align in the structure alignment at all.

Structures in each group were aligned by the multiple structure alignment function of RSmatch with default scoring matrices.

Instead it exploits information on sequential and structural similarity directly by using sequence-structure alignment.

The sequences are aligned by way of multiple structure alignment program.

For all benchmarks, we use four structure alignment tools to generate reference alignments: TM-align (Zhang and Skolnick, 2005), Matt (Menke et al., 2008), Dali (Holm and Sander, 1993) and our in-house structure alignment tool DeepAlign.

An alternative method is structure-based multiple sequence alignment which aligns three-dimensional protein structures and then aligns sequences progressively to the initial structure alignment [22].

The alignments were manually curated for ambiguously aligned sequences compared to the 3D-structure alignment.

Structural superpositioning was accomplished using the secondary structure alignment approach and the program SSM Superimpose.

A multiple sequence alignment based on the structure alignment was created through a companion program, Match → Align.

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