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In addition, PTMs would typically cause only small changes on the surface of a structured protein, whereas large-scale structural changes, such as disorder-to-order transition, can be expected in a disordered region.
The structural basis for deleterious mutations to a structured protein is rationalized through an understanding of protein folding.
Thus, induced-fit mechanisms are important for structured protein interactions with different partners whether the partners are structured or intrinsically disordered.
In contrast, AtGPx6 differs structurally by replacement of helix 2 by a not structured protein domain and a shorter helix 1 (Fig. 16A).
Helices are repetitive secondary structure elements which make up more than 30 40 % of structured protein domains.[ 89] They are stabilized by intramolecular hydrogen bonds between sequential residues (i and i+ n; Figure 7 a).
Cells use ring-like structured protein complexes for various tasks in DNA dynamics.
Similar(11)
We chose the N-terminal 60 aa of Rif2 because in the crystal structure the well-structured protein begins at residue 61.
TTR is a natively β-structured protein that contains two four-β-stranded anti-parallel sheets (CBEF and DAGH) arranged into a β-sandwich (Fig. 5 42,43,44.
Further definitive evidence for a well-structured protein fragment is the large chemical shift dispersion in 2D NMR experiments for almost all of the expected resonances of a 118 residue piece of oxidized CREMP.
Moreover, secondary structure predictions suggest that 67% of the cleavage sites are located in relatively non-structured protein segments, which is consistent with predictions for Calpain 1 and 2. [13] (data not shown) This indicates that the motif adopts a tertiary conformation and is presented in a flexible loop, consistent with the large backbone of the motif.
The majority of the heteronuclear NOE values were ≥0.8, as expected for a well-structured protein.
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