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To model the transition-state structures from the experimental ΔΔG‡int values, we introduced a method for structure perturbation, searching for those inter-protein orientations that best support the experimental ΔΔG‡int values.
Open image in new window Figure 1 Structure perturbation of Csk identified by 1 H- 15 N-HSQC spectra.
Then, the trial DNA is translated using the universal genetic code and a "score" that estimates the protein structure perturbation introduced by the mutation is evaluated.
Here, we use oligonucleotide-directed structure perturbation, probing in the presence of formamide, and in-virion experiments to examine these models.
Under these conditions, anterograde and retrograde transport pathways were blocked [ 22, 23] although this effect could be secondary to the Golgi structure perturbation.
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Rather, local structure perturbations could affect uracil recognition, e.g. by affecting the phosphate recognition by the serine pinch.
The modeling of these effects is further simplified if flamelet structure perturbations by turbulent eddies are neglected.
The ordered sequence of local structure perturbations described above gives rise to a global conformational rearrangement of the transporter molecule from one state (e.g., S1-DAT) to another (e.g., inward-facing).
This conclusion is not dependent on extensive curve-fitting or structural perturbation of protein structure.
A numerical example involving five networked partial differential equations with boundary observation, control, and disturbances and structured perturbations at the boundary, is presented and which provides insights on the control and synchronization of networked PDE systems.
These patterns are (spatially confined) transient responses to confined, spatially structured perturbations of the globally stable homogeneous steady state.
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