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This plasmid was designed as an insertion mutagen that disrupted the structure of the inserted host gene.
The second bacteria made all the proteins and organelles in the so-called "synthetic cell," by following the specifications implicit in the structure of the inserted DNA.
These data verify the genetic structure of the inserted add back (which has subsequently been confirmed by DNA sequencing and comparison to GenBank entry AJ237587.1).
The structure of the inserted state was modelled by using paramagnetic relaxation enhancements obtained by incorporating 5- and 14-doxyl spin-labelled phosphocholine into the micelle.
Specifically in case of integration into heterochromatin region, the epigenetic information is almost regularly reflected in the chromatin structure of the inserted T-DNA, which results in transgene silencing [ 12, 14, 25].
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To date, complications are related to the site of entry for insertion, the site of implantation (aorta, coronary ostia, base of left ventricle), and to the structure and design of the inserted device.
From one to five layers, the grafted materials were mostly replaced with soft tissue, and the structure and volume of the inserted Bio-A were not maintained but were fully absorbed by the tissue.
The length of each product was compared with two sets of DNA size markers 1 kb plus DNA ladder (Invitrogen) and HMW DNA marker (Invitrogen)—to confirm the structure of the insert DNA.
Fig. 1 Synthetic process of a Au-Ag zero-dimensional structure and b Au-Ag one-dimensional structure Fig. 2 TEM images of templates a Ag NPs of zero-dimensional structure: (right) twined Ag NPs and (left) magnified images showing lattice fringes and b Ag NWs of one-dimensional structure and the inserted diffraction pattern.
For the purpose of continued tertiary structure folding, the inserted immutable secondary structure is tagged with its appropriate summed partial charges and hydrophobicity of each secondary structure region.
To preserve the integrity of the courtyard walls, the floors of the inserted structure stop four feet short, permitting the original details to be seen.
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