Exact(4)
This interaction might distort the structure of the hub domain, slightly weakening the lateral interactions in the hub assembly.
(D ) A molecular dynamics simulation was started from the dodecameric crystal structure of the hub domain (PDB code: 2UX0).
We then created a new model in which the β sheet from the crystal structure of the hub domain was retained and the fourth strand was taken from Snrnp, with appropriate changes to the sequence.
For example, we mutated the arginine residues in the hub cavity that we have implicated in the exchange process, but this resulted in a substantial loss of fluorophore labeling at residue 335, indicating that the structure of the hub domain might be compromised.
Similar(56)
(A ) The crystal structure of the dodecameric hub assembly of human CaMKIIγ (PDB code: 2UX0).
(C ) The structure of the vertical hub dimer from CaMKII is shown in comparison to a dimer of NTF2 (PDB codes: 2UX0 and 1OUN, respectively).
(B ) When the trajectory is compared in the same way to the crystal structure of the dodecameric hub assembly, the rms displacements in Cα positions are calculated between the E subunit from the trajectory and G subunit from the crystal structure.
DOI: http://dx.doi.org/10.7554/eLife.01610.021 This decameric structure, derived from the crystal structure of the dodecameric CaMKIIγ hub assembly, was used to initiate two independent molecular dynamics trajectories (100 ns and 50 ns, respectively).
A case study on Wuchang shows the urban traffic network possesses the characteristics of community structure, and the Hub road sections in this network derived from the algorithm is consistent with actual situation.
We estimated the relationship between the network connectivity and its hubs, and found that inhibiting the first 8 hubs had a substantial effect on the remaining sub-network, mainly due to the compact structure of the network where the hubs are also part of many regulated small circles.
We note that using a randomization of the connectivity matrix, preserving degree sequence of the connection and thus the hub structure of the network, ensured the use of a conservative model to estimate the density of connections between >k nodes, and therefore a conservative estimate of rich club organization (van den Heuvel, Kahn et al. 2012).
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