Our results can be interpreted as a quantitative definition of the "similar chemical structure" criterion, based on similar predicted protein-target interactions, which will prevent inactive molecules being prohibited and hence protect athletes against unjust disqualification.
The proportion of clinics that have satisfied the target of each of the structure criterion.
Development of a target secondary structure criterion for oligonucleotide array design is expected to impose restrictions on the probe selection beyond the sequence similarity and melting temperature criteria that are currently used, especially in cases where short probe length restricts the annealing temperature used in the hybridization protocol to 22 37°.
Our folding filter is novel in that it takes advantage of our discovery [50] that requiring both strands of the genomic sequence of a putative miRNA precursor to satisfy Ambros-like secondary structure criteria yields higher specificity for confirmed miRNAs than does the customary requirement on only one strand.
All the 12 followed secondary structure criteria forming a stem loop structure with miRNA sequence sitting at one arm of the hairpin and negative minimal folding energy.
For example, Smith et al. established MASS (Morphology, Attenuation, Size, and Structure) criteria to evaluate tumor response to sorafenib and sunitinib in a cohort of 84 patients [ 51].
Our analysis revealed that in the NC library 2,248,474 sequences can be used for fold-back structure prediction and those that fulfilled the hairpin structure criteria described by Jones-Rhoades et al. [ 1] were selected as candidate miRNAs (Table 1).
By searching all C. trifoliata sRNAs against ESTs and predicting the secondary structures of a series of sequences surrounding them (Additional file 2), we identified 10 sequences that satisfied the secondary structure criteria as established by Zhang et al. [ 29] and shown in Table 3 with all the sequences meeting the new criteria of miRNA annotation [ 10].
Descriptive statistics on standard of care will be calculated by the percentage of clinics meeting the structure criteria, and percentage of subjects enrolled, attended, completed the programme, receiving criterion process of care, investigations and referral per protocol, and percentage of subjects achieving the criterion outcomes of care, in each audit cycle, and at the end of five years.
Descriptive statistics on standards of care are calculated by the percentage of clinics meeting the structure criteria, percentage of subjects enrolled, attendance and completion of the programme, receiving criterion process of intervention, investigations and referral per protocol and percentage of subjects achieving the criterion outcomes in each audit cycle.
Other websites such as PDB-Select [ 2], ASTRAL [ 3], PDB-REPRDB [ 4] and PISCES [ 5] have similar functions, all of which allow users to download a pre-defined chain list or generate a customized list with some sequence or structure criteria.
