This conclusion is not dependent on extensive curve-fitting or structural perturbation of protein structure.
With regard to the two spliced structured segments, the larger structural perturbation corresponded to the omission of a short helix in the shorter splice variant.
However, electron transfer between the [3Fe 4S] cluster and heme in the SdhB-Ile209Ala variant is only slightly perturbed, consistent with a more modest structural perturbation as compared with the SdhB-Ile209Lys variant.
It is well documented that the dehydration step, required before introducing an enzyme to the organic medium (and usually achieved by lyophilization), induces structural perturbation in the enzyme's secondary structure [ 12, 19].
Such a structural perturbation was seen recently in the X-ray structure of related bacterial transporter MhsT33, and has been suggested to be a required mechanistic feature of the NSS transporters to allow release of the Na+/Na2.
Most of them rely on the analysis of the energetic and/or structural perturbation introduced by the variations in the protein native structure.
The slight deformation of the almost cylindrical structure of the DNA double strand is a clear sign of the structural perturbation caused by the different intercalant molecules.
Figure 4 shows the relationship between in vivo fitness and the size of the structural perturbation d ij, computed in silico as the Hamming distance between the structure shown in Fig. 1b and the MFESS obtained for each mutant.
We suspect that the N159V mutation causes a relatively large structural perturbation in the substrate binding pockets.
Activity, aggregation, and structural perturbation were studied in frozen rabbit muscle L-lactic dehydrogenase (LDH) solution.
Biophysical techniques confirm the secondary and tertiary structural perturbation in GHb as compared to native Hb.
