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According to structural discussions, the active sites of LPMOs are held in the center of an extended flat face structure unlike the tunnel-shaped structures housing the active sites of canonical hydrolases (i.e., endo- and exoglucanase)—for an efficient interaction with substrates such as cellulose (including cello-oligosaccharides) and chitin (Hemsworth et al. 2013a; Isaksen et al. 2014).
A number of previous structural discussions (see for example, [ 24, 45, 46] for relevant discussion) have dealt with questions related to the potential persistence of an approximately 10-bp periodicity in sequence accessibility over multiple adjacent conventional nucleosomes.
Overall, these fall into three categories: (A) concerns regarding the presentation and discussion of the functional data, including certain vagueness of the methods; (B) information content of the structural figures and corresponding structural discussions, which I think can be significantly strengthened; (C) putting results into overall perspective, which I feel could also be improved.
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The above structural discussion is based on hydrogen bonding being used to create sheets in two dimensions.
For triamterene, the chemical hydrogen bond connectivity of the first co-ordination sphere may be visualised using MERCURY [19] software to show the hydrogen bonded dimer shown in Fig. 1 and the hydrogen bonded contacts that will form the basis of the next part of the structural discussion (see Fig. 2).
LW participated in the design of the variable length motif models and wrote the structural discussion.
These findings can be reinforced developing software to automate the structural analysis of discussions and actions to the code and documentation.
Some structural properties of discussion threads are: Fig. 5 Example of a discussion thread represented as a tree: at time-step t=9, node (comment) number 10 is added to the thread.
According to these results, we used the complex model with the higher quality for the structural comparison and discussion.
B) Concerns regarding structural figures and discussion: 9) It would be helpful if authors referenced Figure 3 figure supplement 2 early, when describing the overall structure, to make it easier for the reader to follow the discussion.
For a detailed structural analysis and discussion (both of which are beyond the scope of this review), the the original reports are referred to the reader (Bertrand et al. 2010, Labar et al. 2010a).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com