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The combination of a specific sequence signature with its structural location emphasizes the conservation of the closed-loop structure, regardless of the exact secondary structural content of the loop and interaction of this loop with its structural environment.
The analysis of secondary structure revealed that all mutant enzymes presented structural content slightly different to the wild type enzyme (Fig. 3C; Table 3), similar to the observed to AspG and similar to other mutants using a different approach22.
This indicates that the aggregates observed in the presence of 5-HT are partially structured and display a significant beta-sheet structural content.
CD experiments showed that both full-length 27 kDa 7B2 and the C-terminally truncated 21 kDa form lack appreciable secondary structure, although the longer protein exhibited more structural content than the latter, as demonstrated by intrinsic and ANS fluorescence studies.
Studies on the secondary structure by circular dichroism (CD) confirmed that the overall structural content of Ts-PCHTP is predominantly β-stranded (Figure S4).
Extending structural information across uncharacterized protein families represented in the M. tuberculosis proteome, by determining superfamily relationships between families of known and unknown structures, has contributed to an enhancement in the knowledge of structural content.
For the helical peptides and proteins studied herein, structural content per residue was assessed using the Dictionary of Secondary Structure in Proteins (DSSP) [27], which has gained general acceptance among the biophysical community.
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It has been shown that the dipeptide compositions of proteins are important parameters for protein structure and have been used extensively to enhance the prediction quality for protein structural contents and cellular location [ 54, 55].
Finally, the deconvoluted amide I spectra were curve-fitted, and the area ratios of the above assigned bands to the total Amide I area were used to determine the fraction of the secondary structural contents in the films34,35,36.
Floor isolation systems have been becoming increasingly popular as a protective measure for critical structural contents such as computer servers or museum artifacts.
Autocorrelation descriptors can be used for predicting transmembrane protein types, protein helix contents, and protein secondary structural contents.
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