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Taken together, these findings suggest that bacterial populations in unsaturated soils are strongly subdivided and genetically heterogeneous within local environments.
Importantly, this only holds for strongly subdivided populations, as the three sampling schemes lead to similar values of Tajima's D at intermediate and high migration rates (above 4Nm = 5 or 10).
In the simulations by Städler et al. (2009) that only included global expansion, Tajima's D is expected to be near zero or positive for the same levels of migration; in other words, local sampling is detecting signals only at a local level in strongly subdivided populations.
The strongly subdivided population structure increases the effective population size of land snail species and, thus, the likelihood of a long persistence of ancestral polymorphisms.
Our study suggests that ancestral polymorphisms are a frequent cause for nonmonophyly of species with a strongly subdivided population structure in gene trees.
It can be considered a paradigm for one of the causes of the high mitochondrial sequence diversity within land snail species discussed by Thomaz et al. [ 36], namely long-term persistence of ancient polymorphisms resulting from the strongly subdivided population structure of land snails.
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The second clade could be subdivided into two strongly supported subgroups.
In addition, M. stylosa was further subdivided into two strongly supported (100%/100%/100% and 76%/74%/100% in MP, NJ, and BI, respectively) monophyletic clades, with each corresponding to the two taxonomic varieties, M. stylosa var.
These SAFV-2 strains were further subdivided into 2 strongly supported clusters: DK-A, which comprised viruses isolated during 2009 2011, demonstrating probable persistence in Denmark during this period; and DK-B, a smaller group that included viruses from United States, Germany, and the Netherlands, indicating widespread viral gene flow.
Within each of the Sierra Nevada clades there are strongly supported monophyletic groups that are geographically subdivided.
Two major clades are strongly supported (nodes B and D) and these are further subdivided into subclades.
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