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As expected the p63 signal strongly stains the first basal layer of cells and its intensity gradually decreases (Fig. 8A).
The signal for pT67 is not present in late G2 cells (TT+8) but strongly stains extracts from mitotic cells.
7-AAD denotes the lobe interior, while DAPI strongly stains the peripheral rim (Fig. 9c).
In particular, POPO-1 strongly stains the nucleolus and cytoplasm of the germinal cells.
However, we have observed that Alizarin Red strongly stains chitosan membranes also in the pH range of 4.1 – 4.1.
We further find that an anti-phospho-Lgl antibody strongly stains both wild-type and aPKC-null mutant mitotic cells.
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Furthermore, the matrix around the cell clusters was strongly stained in safranin-O-stained sections at 5 weeks post transplantation.
Figure 1 shows representative sample cores of non-staining, weakly staining, intermediate and strongly staining cores.
The weakly stained colonies contained sparse cells, while strongly stained ones had piled-up cells (data not shown).
A), C) Dark blue staining in coleoptile and leaf base, D) E) Strongly stained embryogenic calli and mature seed B), F) slightly stained root and leaf blade.
Histological examination of the tissue revealed it contained a cartilage-like matrix strongly stained with toluidine blue.
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