Immunoblot analysis of serum samples from the 22 animals showed that 2 of the brush mice were strongly reactive with antibody reactivities equal to those of the positive control sample.
In immunohistochemical stainings, the thrombi were strongly reactive for IgM and lambda light chains without reactivity for kappa light chains, IgA, IgG, C1q and C3c.
As a result, epithelioid cells in both primary and metastatic foci showed strongly positive reactivity for progesterone receptors and negative reactivity for estrogen receptors [Figure 1].
In addition, normal pancreatic islet cells showed strongly positive reactivity for progesterone receptors and weakly positive reactivity for estrogen receptors [Figure 1].
We want to emphasize that, as a result of the Au passivation, we are able to create a silicon surface with a strongly reduced reactivity, which is essential for the correct interpretation of our results below.
Furthermore, our immunohistochemical evaluation showed that both epithelioid cells and normal pancreatic islet cells showed strongly positive reactivity for progesterone receptors.
In HL-60 cells used as positive controls, the cell membrane and cytoplasm stained strongly (3+ reactivity) with the CHO-131 mAb (data not shown).
The binding intensity of SSc AFAs correlated strongly with reactivity against topoisomerase I on immunoblots of fibroblast extracts, and with the immunofluorescent pattern typical of anti-topo I on permeabilized cells.
Gold carbenes, because of the weak metal-to-carbene π-back-donation and their strongly electrophilic reactivity, could be classified into the broader family of Fischer carbenes, although their behavior and properties are very specific.
In contrast, p155 175 was strongly correlated with reactivity to the complete protein (P < 0.001) and both reactivities were associated with autoAb to dsDNA and correlated significantly with clinical disease activity, skin involvement and proteinuria (P < 0.01).
