For instance, the epitope RKSVRIGPGQTF on 93MW965.26, which is strongly neutralized by HGN194 (i.e. IC50 <0.02 µg/ml), is shared with Du422.1, ZM197M.PB7 and Du172.17, isolates not neutralized by HGN194.
LT-6E8 alone had little effect except at very high dose; 230-4A2 alone gave some neutralization, possibly by inhibiting infection post-binding; but LT-6E8 and 230-4A2 together were strongly neutralizing.
For IgM, the regression slope was actually negative and not significantly different from 0 (m = −0.163, p = 0.656), suggesting that IgM recognizing EDIIITS epitopes in these sera is not strongly neutralizing.
For example, two clade A viruses that showed strong resistance to AH (Q461.e2 and Q168.a2; see Table 1) were strongly neutralized by the dimer with IC50s less than 1 µg/ml (Tanaka & Matoba, manuscript in preparation).
The three Eurasian lyssaviruses Irkut, Khujand, and Aravan were all strongly neutralized (Fig. 3 C).
Several lyssavirus pseudotypes were strongly neutralized, with the exception of two Duvenhage strains (SA06 and RV131) and two European bat lyssavirus type 1 strains (RV9 and RV20).
Warter et al. described 5F10 and 8B10, two human mAbs which strongly neutralized several CHIKV isolates in vitro, without cross-reactivity against other alphaviruses but to Onyong-nyong virus [ 56].
Today, in a paper published online in Nature Immunology, an independent group at Imperial College London reports similar results, showing that a number of different antibodies to dengue virus react to Zika, but not strongly enough to neutralize the virus.
However, they were able to identify two mAbs able to strongly bind and neutralize all genotypes [ 114].
We surmise that this strongly acidic environment neutralized the surface negative charge of PQDs, resulting in agglomerate invisible to the naked eyes.
