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Using our genetic strategy to label individual mature GCs, we found that the red fluorescent marker, tdT, strongly labels the cell body, axon and dendrites (Figure 4B C).
Antibody against a conserved cytoplasmic epitope found on MHC-II I-Aβ which strongly labels BMDC but not macrophages was used throughout the experiments.
Our results showed that GSK3β strongly labels neurons in the cortex and striatum (see pSer9GSK3β results and figures) whereas in an adjacent section that was processed with the peptide-preadsorbed antibody no immunostaining was observed (Figure 1C).
Besides labeling all five MB lobes by GAL4-OK107, we counterstained MB with anti-Trio antibody that strongly labels α/β lobes and weakly labels γ lobe [52] to make the five MB lobes distinguishable.
We immunostained lung sections with an antibody that strongly labels S100B but exhibits minor cross-reactivity to S100A (S100 antibody).
MNs were labeled with toluidine blue, and immunocytochemically using an antibody to non-phosphorylated neurofilament epitopes, SMI-32, which strongly labels MNs as well as their neuritic processes.
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Previous attempts to use various brightfield double labeling methods did not allow quantitative analysis of all double-labeled thalamo-cortical fibers, because strongly labeled fibers and terminals appear black [103].
They strongly labeled Aβ deposits in post-mortem brain tissue of AD patients and APPxPS1 mice.
Curcumin phospholipid conjugate-based ex vivo liposomes delivery system provides strongly labeled Aβ deposits [54].
Consistent with these results, MVA particles were poorly labeled with antibodies against the surface of intracellular mature virus but strongly labeled with antibodies against an envelope antigen.
Primary human fetal astrocytes were less strongly labeled with 5-CF-Apelin-13, and in primary human CNS MVECs only weak distribution of green fluorescence specific for APJ in the cytoplasm was observed.
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