Real-time RT-PCR demonstrated that the expression of the TPX2 gene was strongly knocked down by TPX2 -siRNAs in the three selected pancreatic cancer cell lines, KLM1, KP4, and Panc1 (Fig. 1E).
An old-fashioned romantic comedy with a contemporary metaphysical twist opened just strongly enough to knock the reigning horror film from the top of the box office this weekend.
The wind blew so strongly that it knocked down tables, and vases full of rocks, and buffeted firefighters and residents in retreat.
Unlike homozygous combinations, which strongly disrupt or knock out one step in the pathway, double heterozygous combinations only reduce Imd signaling at two different points in the pathway.
In line with the results obtained on NF-κB activation, knocking down CHC strongly enhanced basal IL-8 secretion (Figure 3A), showing that, indeed, the expression of CHC was critical to prevent constitutive IL-8 expression in HeLa cells.
Second, in a yciR mutant background, also knocking out the DGC YdaM strongly reduced csgB expression (to the 'basal' level), whereas knocking out the other DGC, that is, YegE, did not produce any effect.
Given the predicted directed signaling pathways, we can estimate the phenotypic effects of knocking down genes by assessing how strongly the downstream TFs are affected.
Furthermore, knocking down p53 with morpholino in shh−/− mutant strongly inhibited expression of p53 reporter transgene at 24 hpf (Fig. 4C).
From that screen, we identified clathrin heavy chain (CHC) as one of the proteins that, when knocked down, strongly enhanced activation of NF-κB.
Still, they stood strongly behind him once he became the Democratic presidential nominee, contributing money, running phone banks and knocking on doors in critical swing states like Florida, Ohio and Pennsylvania.
