We study the evolution of large but finite asexual populations evolving in fitness landscapes in which all mutations are either neutral or strongly deleterious.
Another point about the fitness effects of buffering polymorphisms is that it is entirely possible that dual buffering polymorphisms arise frequently but are strongly deleterious and therefore not present in our panel of strains due to negative selection.
There are two general explanations; first, the proportion of mutations that are strongly deleterious is higher in 18S rDNA, when compared to protein sequences, in unicells compared to multicells.
Our inability to detect a difference between the high and low lines suggests that neither the eye deformity itself nor the genetic variation producing the deformity had strongly deleterious effects.
In exons, even the inframe indels are strongly deleterious.
These findings suggest that genetic variation in the miRNA regulome may have strongly deleterious phenotypic consequences.
Similar large expression shifts for more typical genes would probably be strongly deleterious.
The proportion of strongly deleterious mutations, however, was similar in the two species.
Acetylation of the N-terminus (KYG-178) was strongly deleterious to binding.
To simplify this, in our simulated coding sequences, all nonsynonymous substitutions were considered strongly deleterious and rejected.
Therefore, the gltA1 mutation, while seemingly having little effect on glucose growth, was strongly deleterious in Cit++ cells.
