In probably his most self-critical judgement, Blair has accepted the west did not foresee the degree to which complex tribal, religious and sectarian tensions would be uncorked once a strong, repressive, all-powerful leader such as Saddam was removed.
Similarly, (D' ) MEK/ERK inhibition has a stronger repressive effect on FGF target genes than PI3K inhibition at all thresholds.
Thus, short telomeres have fewer repressive proteins bound and are more frequently elongated, whereas on longer telomeres, the Rap1/ Rif1/ Rif2 exerts a strong repressive effect so these telomeres are elongated less frequently.
Conversely, uAUGs with strong repressive potential (2×-6×) are complementary to expressed miRNAs except for the first uAUG in the ADH5/FDH gene and second uAUG of the KLF13 gene, where expressions of the predicted miRNAs have not been detected.
Furthermore, the transfection assays sustained these data and showed that most notably MBD1v1 (80%) had a strong repressive effect on CD44, whereas MBD2a and MeCP2 had almost no influence on the methylated promoter.
We conclude that two sequence elements of N-Bak mRNA, namely uORF in the 5'UTR and exon exon junction downstream of PTC in the 3′UTR, have strong repressive effects on its translation.
Using this method, we identified several miRNAs with a strong repressive effect (Supplementary file 1), although others that are naturally highly expressed in HEK293 cells even without transfection may have been missed.
Most of the miRNA candidates that had a strong repressive effect in the screen with the PTC- APC reporter (Supplementary File 1), such as miR-138, miR-29a, miR-127-5p and miR-378, have very low or undetectable expression levels in HEK293 cells (in-house sequencing data).
