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The strongest regulated genes in the kidney cortex and medulla, spleen, and liver were Dao1, Cfd, and OTTMUSG00000007485 transcript, respectively.
Galectin-3 was the strongest regulated gene, being up-expressed in decompensated hearts more than fivefold compared with compensated hearts.
Interestingly, both genes were immediately (5 min after treatment) and constantly upregulated after carolacton treatment and belong to the strongest regulated genes of our time-resolved transcriptome study.
A rat model of HF revealed that galectin-3 is the strongest regulated gene when compensated left ventricular hypertrophy was compared with overt HF (4).
Among the strongest regulated genes we found five genes (Dkk3, Hoxd8, Hoxd9, Hoxd10 and Tbx1) which are especially known for their critical role during embryogenesis.
Aiming to further elucidate genes that facilitate the proposed function of CAR in cancer, we determined differential gene expression after CAR knockdown in the human colon cancer cell line DLD1 and assessed the functional impact of the strongest regulated gene α-catenin.
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Most genes were differentially expressed at both time points, but a significant number was stronger regulated at the later time point of impedance change.
Of those, 38 were stronger regulated in CA3 24 h after ischemia, and 59 in CA1.
The sucrose cleavage enzymes cell wall-bound invertase and sucrose synthase had very different expression patterns with Susy 4 being much stronger regulated.
However, among these genes, the expression of only a few was significantly stronger regulated after 24 h than after 6 h of both TSA and BMP2 treatment.
The selection of these groups was either based on major differences between oxygen supply conditions or on the potential impact on protein production, as some of them appeared to be generally stronger regulated in the Fab producing strain.
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