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Moreover, initial cell adhesion did not differ significantly between them, while the strongest proliferation was observed on the hydrogel with the highest degree of cross-linking.
Notably these cytokine combinations induce the strongest proliferation and differentiation from purified KLS cells.
Expectedly, joint stimulation of BCR with TLR7 triggered strongest proliferation in our present study.
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However, when Rad52 is depleted by shRNAs in FANCM KO cells, a strong proliferation defect is observed, suggesting a synthetic lethality interaction between these two genes.
In this study, we found that epithelium-like MCF-7 could obtain stronger proliferation ability when they were co-cultured with AHFs and CAFs but not NFs.
iTSCM cells showed strong proliferation upon TCR restimulation with resistance to cell cycle arrest and apoptosis, which suggested that iTSCM cells were more likely to be applicable to cancer immunotherapy8,24.
The current study demonstrated that MCs possessed strong proliferation ability but accompanied with rapid loss of chondrogenic ability during passage, indicating a poor feasibility to engineer the entire ear using expanded MCs.
In contrast to MCF-7 alone (MCF-7) or MCF-7 co-cultured with NFs (MCF-7/NFs), MCF-7 co-cultured with AHFs (MCF-7/AHFs) had stronger proliferation potential than that of MCF-7 and MCF-7/NFs, although it was weaker than that of MCF-7 co-cultured with CAFs (MCF-7/CAFs) tested by cell number (Fig. 2b) and cell cycle analysis (Fig. 2c).
Taken together, bFGF-ADM guided similar bone regeneration to BMP-2 through more efficient recruitment of MSCs, and moreover, BMMSCs pretreated by bFGF showed stronger proliferation at 1 5 days and osteogenic differentiation potentials at 14 days compared with BMP-2 pretreatment.
Inoculation of the engineered bacteria into a microalgal culture in the absence of sources of C and N other than N2 and CO2 from the air, resulted in a strong proliferation of microalgae that was suppressed upon addition of the inducer.
Cytology of pancreatic juice showing large cell clusters with small nuclei and strong proliferation, indicating a suspected adenocarcinoma.
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