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The crystal structure of RpfB catalytic domain in complex with N,N′,N″-triacetyl-chitotriose, as described here, provides the first, to our knowledge, atomic representation of ligand recognition by RpfB and demonstrates that the strongest interactions are established by the N-acetylglucosamine moiety in the central region of the enzyme binding cleft.
In case of L-Asp, the strongest interactions are those of O…H type constituting the highest fraction of 72.7%.
Each data point in Fig. 17 corresponds to a specific cluster size and represents the difference in energy sums between the indicated polymorph and form III. As mentioned above, HBSs dominated by t-connections (I III, X) are favoured if only the strongest interactions are taken into account.
It has been clearly shown that the strongest interactions are established between lateral chain atoms of MHCII molecules and atoms from the peptide's backbone (Figure 3), whereas in the peptide/TCR interaction the strongest bonds are established with the peptide's lateral chain atoms (Figure 4), among which salt bridges are the most important electrostatic forces.
Notably, the strongest interactions are autoregulatory.
The five strongest interactions are shown in Table 2.> -wrap-foot> The corresponding gene for each SNP is also shown.
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The strongest interactions were found between width and depth (17.0) as well as width and conductivity (16.9).
PON1 Q192R exhibits substrate specificity (Richter et al. 2009), but our strongest interactions were for dimethylphosphates, not diethylphosphates (into which chlorpyrifos and diazinon both metabolize).
One of the strongest interactions was for cold hardiness, between two SNPs separated by only 61bp in the xth1 gene (SNPs 289 and 350).
Gene set enrichment found that genes involved in the strongest interactions were significantly enriched for transcriptional regulation, apoptosis, cell cycle regulation and response to tumor cells.
In terms of parameter interactions, strong interactions are found among parameters in predicting Ce.
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