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The strongest gene expression signature observed is the down-regulation in tumors of many genes with muscle contraction functions (Figure 3e,f), indicating that the development of this cancer may involve the reversal of muscle cell differentiation found in the oral tongue.
HexA and HexB genes were characterized by the second strongest gene expression in all samples.
HexA subunit gene, one of the two genes encoding for the alpha or the beta chains of hexosaminidase, was characterized by the strongest gene expression.
To circumvent this problem we combined the strongest gene expression patterns into a new matrix and re-clustered them by using the second clustering step, importantly, against the same tumor cohort (Additional file 2: Figure S1 A-D and Figure 1).
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In the present study, instead of using a constitutive promoter, the Arabidopsis ANR promoter (known to drive strong gene expression in the seed coat) was used to express PsLAR in the Arabidopsis seed coat [ 27].
The transition between the two phases is the result of strong gene expression changes (Grupe and Gottschalk 1992; Girbal et al. 1995).Nowadays, with the depletion of oil, renewable processes to replace fossil carbon need to be developed.
In REC1 cells due to even stronger gene expression responses the overlap was even more extensive.
Through the analysis of GFP expression we have previously demonstrated that the eef1a promoter drives constitutive and strong gene expression in all life cycle stages, including liver stage parasites [39].
Firstly, NAT1 was reintroduced into the NAT1 knock-down cells using a plasmid with the NAT1 gene under the control of the CMV promoter to induce strong gene expression.
Since MCF-7 is a cancer cell line, there was a concern that high genome copy numbers in the cells may result in strong gene expression in the absence of HRG that may potentially obscure any HRG-related effects.
However, since MCF-7 is a cancer cell line, there was a concern that high genome copy numbers in the cells may result in strong gene expression in the absence of HRG that may potentially obscure any HGR-related effects.
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