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The strongest expression levels of SRF3 are concordant to those of SRF1, i.e. in experiments investigating programmed cell death, pectin biosynthesis, control of lignification and tumor development.
Additionally, our investigation of the other members of the SHH pathway in the developing cerebellum revealed strongest expression levels in the EGL and IGL suggesting a SHH pathway activation in these respective cerebellar areas.
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Similar to the A2A receptor, CD73 shows its strongest expression level in the CNS within the choroid plexus epithelium and is also detected on glial cells of the submeningeal areas of the spinal cord [ 22, 27, 43].
Five cell lines exhibited relatively strong expression levels of Cyr61, while 2 cells lines expressed lower levels of Cyr61.
In general, the most common plasmid used in recombinant engineering of E. coli is the pET system, owing to its strong expression levels.
After multiple passages in the presence of LIF, cells still maintained the same morphology and strong expression levels of Oct3/4 and another pluripotency-specific transcription factor, Nanog [37], [38], whereas differentiation-related genes such as snail and N-cadherin involved in epithelial-mesenchymal transition were repressed (Figure 3B and data not shown).
uceA1.7 shows strong expression levels, equaling or surpassing the expression levels of CaMV35S.
CK2α and CK2α′ proteins were simultaneously detected and demonstrated strong expression levels.
Due to the strong expression levels of HSP90 in L428 cells siRNA transfection was performed twice by nucleofection.
Analysis of gene expression using RT-PCR has shown very strong expression levels in Capan-1, and undetectable levels in Panc-1 and MIA PaCa-2 cells.
Most genes coding for enzymes involved in amino acid metabolism showed strong expression levels, as expected given the central role of amino acids in embryonic development [ 49- 52].
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