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Therefore, PNA can highly discriminate mismatched DNA and has a stronger binding affinity for complementary DNA than does its DNA counterpart.
Surface plasmon resonance analysis and luciferase assays showed that the T allele demonstrated a stronger binding affinity for miR-25 and miR-32, resulting in significantly weaker reporter expression levels.
The higher docking score of RA indicates stronger binding affinity for caspase-1.
For one, fetuin-A is known to display much stronger binding affinity for calcium (calcium-binding constants of 0.95×10−4 M for BSF and 1.24×10−4 M for HSF) than albumin (7×10−3 M for BSA) [56].
The results revealed that IAH had a stronger binding affinity for human ApoE4 protein than PHC.
The aptamers showed a stronger binding affinity for PS liposomes than for PC liposomes).
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This is contrary to the studies described above for designed peptides and proteins, in which thiolate ligands result in stronger binding affinities for Zn II) than His ligands, therefore highlighting both the importance of the evolved metal site structure in a native protein and the difficulty in examining separate factors for metal site structure using a native metalloprotein.
Additionally, molecular docking results showed a strong binding affinity for compounds 1 3 through chelation between copper metal and ligands.
We further performed detailed analysis on the amino acid contributions to the binding energies and found that the strong binding affinity for 6F-tricin to CDK9 is attributed to the specific binding orientation of 6F-tricin in the ATP-binding site.
As a DNA cleavage domain, we chose an analogue of the metal-binding loop (12 amino acid residues), peptide P1, which has been reported to exhibit a strong binding affinity for a lanthanide ion and DNA cleavage ability in the presence of Ce(IV).
In this process, a water-soluble polymer with strong binding affinity for the target metal ions is added to contaminated water.
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