PA generates a highly reactive nitrene that rearranges to an intermediate with strong reactivity preferences.
In addition, self-reactive serum IgG from 90% of CM patients displayed strong reactivity with the brain proteins found in band-0 of the Panama Blot.
The reagent was found to have strong reactivity to both isoforms (Fig. 4C, D).
There is strong reactivity for the cytoplasm of basal membrane, confirming an intact layer of epithelial cells without invasion.
Serum and CSF IgGs of both patients showed strong reactivity with the nuclei of rat central nervous system (CNS) cells and proteins extracted from neuroblastoma cells.
Such a strong reactivity is most likely due to the formation of dangling bonds and defects as a consequence of the hydrogen depletion caused by the electron bombardment.
Some such as mouse, rabbit, guinea pig, bovine and goat, showed strong reactivity with human IgG4.
Both Omp3 and Omp7 demonstrated strong reactivity towards pooled patients' sera (Fig. 3C).
The anti-serum directed against exon 6 of Fmn1 showed very strong reactivity to actin (43 kDa) with very little specific activity to Fmn1-IV (165 kDa).
Out of five bat sera tested (Figure 1), only sample #49 showed a clear and strong reactivity at a serum dilution of 1∶100.
Controls using HSA as antigen again showed strong reactivity by a single band of 66 75 kDa (Fig. 18B, lane 3).
