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For example, the ENCODE RNA-seq standards [ 20] suggest that transcriptome profiling of polyadenylated positive samples require modest depths of sequencing, such as 30 million PE reads of length >30 bp, while for novel transcript discovery and strong quantification of known transcript isoforms deeper sequencing is required.
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Figure 5C provides the strongest support, but no numbers are given, and it would be stronger with quantification from many animals and at multiple AP planes.
The strong reliance on quantification and the accompanying disregard of experiential knowledge is reflective of the pursuit of objectivity.
Methods, which rely on the quantification of strong "all-out" ash pulses alone, ignoring the weaker pulses would seriously underestimate the actual MER.
In contrast to the previous study, there was a strong correlation between regional quantification of the PET image and regional Aβ density by immunohistochemistry at autopsy.
Quantification showed strong correlation between thrombin activity and fibrin deposition (R =0.55, P <0.001, F test), increased microglial activation (R = 0.35, P <0.01, F test), and the size of demyelinated lesions (R =0.52, P <0.01, F test) at disease peak.
- Though it may be difficult to directly utilize these fluorescence-based techniques in phototrophic organisms that have strong chloroplast auto-fluorescence, quantification of the cytoplasmic redox potential is clearly the next challenge.
We determined the capacity of the peptides to inhibit infection of CD14+ monocytes by HIV-1JRFL, a monocyte-tropic HIV-1 strain, using PCR quantification of strong-stop proviral R/U5 long-terminal repeat structures [14].
The effect of peptide administration on HIV-1 replication in designated experiments was assessed by PCR quantification of strong-stop proviral R/U5 long-terminal repeat structures as described by Cole et al. [14], with minor modification.
There is a strong correlation between Phadiatop quantifications and the sum of the individually measured specific IgE antibodies to the allergens included in the Phadiatop assay [ 12, 13].
For example, the tasks assigned by higher-level party committee and the government still have a strong incentive even though the quantification of reward and punishment is low.
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