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We found strong PLA signals in VX-809-treated Cftr (ΔF508 /ΔF508) organoids (5 μM VX-809 for 24 h) and very weak signals in the untreated organoids.
This staining pattern contrasted with both the homogeneous immunofluorescence pattern observed for total Tau (Fig. 3c) and the strong PLA signal for Tau and tubulin (a major Tau partner) (Fig. 3d).
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The assay detects a strong Fab-PLA signal on resting B cells that was lost upon B activation.
However, compared with the Et value (4 GPa) of polyethylene where only van der Waals interaction acts, there exist no strong intermolecular interaction for PLAs and scPLA.
We first designed specific fluorescent PLA nanoparticles exhibiting strong colloidal stability after encapsulation of either 6-coumarin or CellTrace BODIPY® before monitoring their transport through mucosa in the mouse ligated ileal loop model.
FTIR analysis revealed that there were strong hydrogen bond interactions between the PLA and chitosan component.
MG-63 cells attached well and spread actively on the mineralized PLA microspheres, indicating their strong potential in bone tissue engineering.
The strong interaction resulted from stereocomplexation in PLA based copolymers could be exploited not only for fabrication of advanced therapeutic delivery carriers and tissue engineering devices, but also for stabilizing colloidal systems in microparticles, micelles and hydrogels, that further broaden the applications of PLA that could not have been attained with single PLLA or its copolymers.
In particular, PLA-based NPs are characterized by the slower release (only about 60% of Dtx is released within 6 h) that can be attributed to the stronger hydrophobic interaction between hydrophobic domain of PLA and drug.
The effect of the strong shear flow on the crystallization kinetics of PLA was also examined.
Due to its weak mechanical properties, chitosan has been mixed with PVA, PLA, PEO, or PA to develop stronger ECNFs.
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