Moreover, 50% reduction in the expression of Ku80 either by small interfering RNA (siRNA) or by functional inactivation of one allele in two telomerase-positive carcinoma cell lines, HeLa and HCT116, is sufficient to alter telomere homeostasis, as demonstrated by rapid telomere shortening and, at least in one of the studies, stronger telomere overhang signal [ 16, 18].
The rationale for mild termination is as follows: Termination must be strong enough to create 5' overhangs in the first PCR reaction, but weak enough to allow the polymerase to continue through the modified nucleotide during the second round of PCR (Fig 2).
It descended into a low valley with a strong current that whipped past an overhang.
The 2008 forecast is boosted by an overhang from this year's strong growth and by some extra working days (February 29th, for example).
A fissure's location can prevent you from using the stone if cuts will result in the weakest point's being located where the stone needs to be strong, like around a sink or on an overhang.
In the United States, where mortgages account for most of the private debt overhang, the federal government should enact stronger measures to reduce principal balances on troubled mortgages and to make refinancing easier.
The companies have also stressed a strong desire to reduce government ownership, arguing that the overhang has deterred private shareholders from investing.
There were other mistakes, too: the effort to reduce the overhang of household debt should have been stronger.
Most species avoid locations with strong currents or waves, and typical sites include rocky overhangs, crevices and caves, steep slopes of continental shelves, and in the bottoms of deep oceans.
Moreover, while TUT7 and TUT4 showed a strong oligo-uridylation activity on pre-miRNAs with a long 5′ overhang (pre-let-7a-1 ΔCUUUC and Ac-pre), TUT2 did not show such activity (Fig 2B and Supplementary Fig S2D).
