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DS specifies the dominant site of pre-RC assembly in whole and mini-EBV genomes and is a strong initiation site in the mini-EBV system.
The same, strong initiation site at position 10 was also confirmed by primer RATZEB4 which is positioned upstream of the RATZEB2 (band 127, Figs.3b, 3c).
Using an expression cut-off of four TPM to focus on strong initiation events, 13,970 TCs were retained, of which 92% overlapped an annotated RefSeq gene model.
Whereas two of the alternative AUG codons appear incompetent for translation initiation, we detected strong initiation at the third AUG, which is followed by one AAA codon and a UAG stop codon.
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These data indicate that oriP is the strongest initiation sites present in the mini-EBV episomes (Fig. 5A, B).
Despite its shortcomings, this study provides a strong foundation for the initiation of screening programs for STDs in this high-risk inmate population in Saudi Arabia.
For example, Notch-GFP reporter system has been used to separate CSCs from lung adenocarcinoma and breast cancer, GFP+ cancer cells could differentiate into GFP− cancer cells, and have strong tumor initiation capacity (D'Angelo et al., 2015; Hassan et al., 2013).
We demonstrated that TISU, which has an invariable ATG, composes a strong translation initiation context.
Given the strong translation initiation capacity of TISU, it is likely that in these genes it competes with the downstream AUG, and behaves as a strong inhibitor of translation.
These results suggest that in addition to the −3 and +4 positions of TISU, sequences in the other positions contribute to its strong translation initiation activity (see also Fig. 5).
We show that translation in vitro and in vivo is initiated exclusively from the AUG of this motif, and that the AUG flanking sequences create a strong translation initiation context.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com