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The two control samples, 1078-A1 and 1153-A1, distrongd stronuclearear staining and strong foci staining was also detected in 1153-A1 (Supplementary Figure 5B).
Twenty-five percent of the samples displayed strong multiple nuclear foci staining with the remaining samples displaying only one or two strong foci per nucleus.
Homozygous version (eGFP-Trf1KI/KI with eGFP-Trf1KI/KI withy—, heterozygoustrongion (eGfocirf1+/Kintensityockdown for TRF1 (sheterozygous low foci intensity—were used to versione this system (Supplementary Fig S7B).
γ-H2AX, which is a maker for DNA damage, was found to be expressed in all three treatment groups, but strong foci were only seen in the combination group.
Ago2 staining showed the typical diffuse punctuate pattern throughout the cytoplasm with the typical enrichment in the perinuclear region and a half-moon shaped structure (Golgi) and some individual strong foci (P-bodies).
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