Immunostaining of each section was semiquantitatively scored for both intensity (1, absent/weak; 2, moderate; 3, strong) and extent of staining (percentage of the positive tumor cells: 1, < 10%; 2, 10-50%; 3, > 50%).
The scoring system of triplicate tumor cores included intensity of staining (0, none; 1+, weak; 2+, moderate; 3+, strong) plus extent of staining, which was equal to the number of cores with a positive staining (extent 0, no staining in three cores; 1, only one core had a positive staining; 2, only 2 cores had a positive staining; 3, all three cores had the positive staining).
The strongest extent and frequency of downregulation were shown for ALDH1L1 gene both in ccRCC and papillary RCC.
As expected, there is a strong correlation of extent of fibrosis with the degree of initial injury.
As with breast cancer, there is strong evidence that extent of disease at diagnosis (stage) is a major prognostic factor in patients with colorectal cancer.
Immunostaining was scored on a 3-tiered scale for both intensity (absent/weak, 1; moderate, 2; strong, 3) and extent of staining (percentage of positive tumor cells < 10%, 1; 10–50%, 2; > 50%, 3).
Semiquantitative classes were used to describe the intensity (absent, 0; weak, 1; moderate, 2; strong, 3) and extent of staining (percent of positive tumor cells: absent, 0; < 10%, 1; 10-50%, 2; > 50%, 3).
But Senator Bill Frist, a Tennessee Republican, heart surgeon and a leader on health issues, said, "President Bush has done the nation a great service by allowing promising embryonic stem cell research to proceed while maintaining strong restrictions on the extent of the research and ensuring comprehensive, public oversight".
In addition, we identified a strong correlation between the extent of creep in the material and its crystallinity.
Population growth, especially in rural areas, had a strong effect on the extent of overall land degradation.
