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The use of oligonucleotide adaptors that form strong clamps ensures the specificity of the method, such that only fragments with two different adaptors will be amplified, whereas fragments with one type of adaptor will not be selected for amplification due to the suppressive effect on PCR of the adaptors [ 36].
You can also use strong clamps if you don't want to or don't have weights.
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This value is sufficient to ensure a dynamical response of the network without a strong clamping effect.
Under these same assumptions, the strong clamp binder, QLSLPL, would remain bound to the β clamp, preventing access by the translesion polymerase.
With the strong clamp-binding mutant (QLSLPL), the tripartite complex is tightly associated, showing even higher processivity than the wild-type complex (Jergic et al. 2013).
This parity is thought to arise due to the presence of a mechanically strong clamp between the complementarity determining region in the antibody domains and the point of force application that prevents any force-induced remodelling of the complex.
For example, LFKQL behaves similar to the strong clamp binder; it is a low mutator (consistent with preventing Pol IV access to the clamp), SOS constitutive, and able to induce SOS after nalidixic acid at levels even higher than that of wild-type.
If, after the glue has dried, you can pull off or move the wooden fins, repeat with a stronger glue or a stronger clamp.
By the end of 1937, however, the dictatorship, fearing that the opposition was growing too strong, had clamped down on political activities.
QLSLPL, the strong β-clamp binding mutant, retains catalytic proofreading function as expected but showed a basal SOS level significantly higher than wild-type (P = 0.0000134) and closer to the ε knockout.
It must be near the edge of a table on which you can hook a strong C-clamp.
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