Recent studies reported that the East Asian type CagA containing segments EPIYA-D exhibits stronger binding activity for SHP-2 and a greater ability to induce morphological changes in epithelial cells than Western type CagA containing segments EPIYA-C [17], [20], [23].
As a result, East Asian-type CagA exhibits stronger binding activity for SHP-2 and a greater ability to induce morphological changes in epithelial cells than the Western type.
The only strong binding activity of MBP-FYVE was observed for the monophosphoinositide PI(3 P (Figure 4A).
As the binding ability of Con-anc-N/C/L5 was only 30%-40 30%-40at of ConI (described later), the other structural elements responsible for the strong binding activity of ConI, namely, L6 and L3 (Thr38), located at the carbohydrate-binding cleft, were also investigated.
Unlabeled DQA1*0101 oligonucleotides successfully competed for strong binding activity seen in nuclear extracts from the Namalwa B cell line (Fig. 3a, lanes 3 and 4), whereas unlabeled DQA1*0501 Y-box sequence competed less well (Fig. 3a, lanes 5 and 6).
Immunofluorescence staining showed that hHP-1 had a strong binding activity to SMMC cells.
On the other hand, mutant protein containing only RRM1 exhibited strong binding activity to the JCV promoter (Fig. 6C).
The result shows that RNODN has specific and strong binding activity on the NF-κB protein.
Taken together, our HS-binding CPP ecp possessed strong binding activity to tumor cell surface with higher HS expression, while depletion of cell surface HS abolished such highly selective binding activity of CPP ecp to tumor cells.
Four positive sdAbs with strong binding activities were obtained, expressed in Escherichia coli and purified.
