Low-pH acid washes have been employed to strip cell surface-bound 12G5 and chemokines in flow cytometric assays.
He strips cells from this tissue and adds keratinocytes-the cells that make up the top layer of skin-from the foreskins of circumcised baby boys.
Purity of stripped cells was confirmed by immunostaining for SC markers 97±1% of stripped cells was positive for Pax7, 92±8% for Myf5 and 95±3% for MyoD after adhesion (500 cells examined for each marker, 10 random fields evaluated at 10× magnification, 5 different experiments; Fig. S3).
Published: June 4, 2015 We previously showed that protoplasts of B. subtilis, derived by stripping the cell wall with lysozyme in the presence of an osmoprotective agent such as sucrose, fail to proliferate in this state [ 7].
If you got sent down, the screws weren't interested, they'd sling you in a strip cell and left you till you calmed down, which could be days.
"If I even whispered to the man in the next cell [this] resulted in being beaten by guards, sprayed with chemical mace, black jacked, stomped, and thrown into a strip cell naked to sleep on a concrete floor without bedding, covering, wash basin, or even a toilet.
The five cells of "The Hole" had nothing but a sink and toilet and the very worst cell was nicknamed "The Oriental" or "Strip Cell", the final cell of the block with nothing but a hole in the floor as a toilet, in which prisoners would often be confined naked with nothing else for two days.
DOI: http://dx.doi.org/10.7554/eLife.03115.006 We asked whether the Casparian strip cell wall defect is caused by a defect in the formation of the CSD, or whether the function of SGN3 lies downstream of the CASPs, as has been demonstrated for the NADPH oxidase SGN4/RBOHF (Lee et al., 2013).
Soluble heparin can also rescue signaling behavior in cells that have been stripped of cell surface HSGAGs.
