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Number of melanophores in the marked light stripe area is shown in the upper right corner.
We calculated the DAPI covered area by subtracting the summed area of all nuclei from the total stripe area.
Several zebrafish mutants including leopard (Haffter et al., 1996; Watanabe et al., 2006), luchs (Irion et al., 2014), and seurat (Eom et al., 2012) exhibit a spotted pattern formed by ingressions of iridophores into the dark stripe area.
S-iridophores attract melanophores in high numbers, induce their aggregation into the prospective stripe area (positive long-range effect) (1) and are to some extent able to exclude them from the interstripe (negative short-range effect) (2).
Similar(56)
In wild type, dense iridophores normally stay restricted to developing light stripes, but occasionally spread into the prospective dark stripe areas.
In older mutant fish, there is a slight decrease in the average number of melanophores, likely due to the interruptions of the stripe areas.
Naturally, we do not state that abnormal cell movement is the only possible cause of the iridophores invasion into dark stripe areas.
In summary, we hypothesize that the loss of Tjp1a impairs the ability of iridophores to recognise the (as yet unknown) cues defining the dark stripe areas or their ability to react to them efficiently.
The failure to precisely form the boundary between light and dark stripes might be a cause for another anomaly observed in sbr mutants: L-iridophores, which are restricted to dark stripe areas in wild type, were observed in light stripes of adult sbr mutants.
Stripe domain.
Therefore, we compute the mean of the gradient magnitude in a stripe-like area within a fixed distance to the contours of the detected specular regions.
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