To further investigate the effect of MAL overexpression on gene transcription, microarray data were analyzed more stringently using an FDR-adjusted p value of < 0.05.
We were particularly interested in generating a tool that would reliably identify a patient in a 'quiescent' state, defined stringently using a composite of criteria including physician rating and treatment outcome.
Insomnia may be defined as simply self-reported "difficulty sleeping" on one or more nights in the previous month or more stringently using, for example, the DSM-IV criteria of primary insomnia which excludes insomnia associated with psychiatric or physical illness [ 1- 8].
The phosphorylated AR antibodies were stringently validated using western blot and peptide competition assays.
The same associations between HPV loads and persistence were obtained when HPV persistence was defined more stringently by using three consecutive HPV-positive visits for the same type.
When these RNA-Seq data were stringently filtered using four parameters (Fold change > 5, RPKM average > 1, P-value < 0.1, non-pri-miRNA), we identified 528, 473, and 392 3′ extension candidates in xrn3, xrn2xrn3, and xrn3xrn4, respectively (Table 1 and Table S3).
Therefore, the current study used stringently defined ER α-negative tumours and so was enriched for a generally more aggressive group of breast tumours.
The laws are there, but they are not always used as stringently as they might.
These data indicate that in the absence of known dates of infection, CD4 slopes can be used to stringently delineate participants into distinct disease progression groups.
SAM was used to stringently select (FDR<0.05) statistically significant genes.
