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To stringently identify human-mouse orthologs, we selected genes whose official gene symbols were identical between humans and mice.
The "lookahead test" and the "third alignment going inwards" options were also selected for the genome processing in order to more stringently identify IR sequences.
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The stringently identified TF-binding interactions reported appear robust, and provide a starting point to extend focused TF studies into primary hematopoietic progenitors, where ChIP-seq remains technically challenging due to cell-number constraints.
The 22 stringently identified genes were evaluated with the online software Database for Annotation, Visualization and Integrated Discovery of the National Institutes of Allergy and Infectious Diseases; this software identifies groups of genes that are overrepresented using an unbiased algorithm based upon the Gene Ontology classification (Huang da et al. 2009).
It is also possible that the mouse models have been searched more stringently to identify relevant phenotypes proposed by candidate genes.
In order to identify stringently controlled genes as precise as possible, a filtering of the genes appearing differentially regulated in the microarray experiments was performed and three classes were established.
Updated and stringently maintained database helps to identify deficiencies in practice and provides a direction for future improvement.
15, 16 In this study, we use stringently filtered microarray data to identify and characterize transcriptional changes that are associated with kidney transplantation and acute allograft rejection.
The strategy used was to purify stringently for the connexin channels, and then identify lipids that remained associated with them.
To identify PSR-specific transcripts, we stringently excluded all transcripts that had any matches to WT transcripts.
Herein, we stringently designed a four-phase study to identify a panel of circulating miRNAs as early diagnostic biomarkers of GNCA and evaluated their clinical usefulness in independent samples.
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