More stringently, considering genes with paralog (duplicates) may have different evolutionary rate compared with the genes without duplication (singleton) [ 37], a singleton reference coding sequences (s-CDS) was generated as the second reference set, which was composed of singleton genes only.
An adjacent pair was considered stringently conserved if the adjacent relationship was preserved in all five yeast species.
Despite evidence for differential requirement in growth essential genes [9], and initial efforts at scoring essentiality [2], [10], there has not been a quantitative method to determine the expression requirement for essential genes so that only stringently required targets are considered further for drug development.
SNPs were more stringently compared between alignments, and were considered called correctly if both their position and base call matched.
When considering the set of 169 genes considered for functional analysis (i.e. the stringently defined dataset with ribosomal genes removed), we found 101 genes containing coding fRNAs had an EFP greater than 0 (which would correspond to a 40% false positive rate).
In response, the European Union is now considering more stringently regulating how manufacturers label where their products are made.
As the sampling procedure stringently followed the inclusion and exclusion criteria that emphasized the three generations without any different ethnic admixture rule for an individual to be considered as a valid subject for this study, we assumed that there was no recent admixture or gene flow among all the studied populations.
Considering that the liver is the largest internal solid organ and accounts for about 6% of body weight, a balance must be struck during decellularization to stringently remove the cellular component, while at the same time being sufficiently delicate to preserve the ECM.
Their anonymity and confidentiality was stringently maintained throughout the study.
To use so few crosses, the parents were stringently chosen (for methods see [ 10, 22]).
