Like I-DmoI, H-DmoC2 is thermostable and cleaves the new target DNA to generate the predicted 4 nt 3′-OH overhangs but, unlike I-DmoI, H-DmoC2 retains stringent cleavage specificity when substituting Mn2+ for Mg2+ as co-factor.
Human efficacy trials are substituted with a very stringent, possibly more difficult, FDA approval pathway: the Animal Efficacy Rule, which requires a good understanding of mechanistic knowledge in two or more animal models predictive of human response [ 163].
More importantly, the ability of TOG2 to substitute for TOG1 in this more stringent, dimerization-impaired background strengthens the conclusion that the polymerase does not require different TOG domains for its function.
Shale gas is another high-carbon fossil fuel - it just adds to the problem - in the absence of a stringent limit on total carbon emissions it will not substitute for coal.
Britain this year imposed stringent new rules stripping logos and bright colors from cigarette packs, substituting generic lettering, warning labels featuring horrific photos of diseased human organs, and a nausea-inducing color scheme.
All infant formula labeling will have to comply with stringent new guidelines intended to help parents to understand that powdered milk substitutes for breastmilk are not sterile and can contain "pathogenic micro-organisms".
To evaluate the robustness of the effect across varying definitions of infrequent or rare deletion, we repeated this regression analysis twice, each time substituting a measure of CNV length based on a different, more stringent definition of rare CNVs.pecifically, we calculated length using criteria for rare CNVs of "≤3%" and "≤1%".
With more stringent legislation on brominated flame retardants, it is expected that increasing amounts of substitutes would replace polybrominated diphenylethers (PBDEs).
The fact that Cys reversions occurred at these positions to a major extent indicates that site-saturation mutagenesis and stringent mutant selection was able to detect and successfully replace the Cys positions that were more difficult to substitute.
Rationing must be stringent.
The company was among the first to set stringent guidelines for child-friendly gaming, like changing blood color from red to green or, more curiously, in Wolfenstein 3D, substituting rats for guard dogs as shooting targets.
