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The need for a more stringent solvent suggested that the biofilm matrix around the mutant is composed partly of a protein component.
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Due to progressively more stringent organic solvent legislation, the use of supercritical fluids especially those derived from carbon dioxide and water is rapidly developing.
In general, large banks of a stable protein scaffold are generated by randomization of amino acids in solvent-exposed areas, followed by a stringent selection procedure to retrieve binders from such libraries with desirable characteristics.
More stringent rules regulating the use and disposal of organic solvents are increasingly restricting chemical synthesis alternatives, and there is an urgent need to develop non-hazardous alternatives in order to achieve green sustainable chemistry [1, 2].
Although PPCheck showed significant capabilities in differentiating the native-like docking poses from the non-native-like ones in a stringent dataset such as CAPRI decoys, we included residue conservation and solvent accessibility as additional parameters to further improve the accuracy of differentiation.
For instance, antibodies may be generated and selected based on their ability to bind specifically an analyte under predetermined stringent or denaturing conditions (acidic or alkaline pH, chaotropic salts, organic solvents, detergents, high temperatures, etc).
Such arrays of charged beads form under stringent conditions of low ionic strength in aqueous suspension or in other solvents.
In contrast, the restrictions on the P3 site are not as stringent because the corresponding binding site in the thrombin molecule is broad and exposed to the solvent.
Rationing must be stringent.
To die solvent.
"So it's solvent".
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