Sentence examples for stringent solutions from inspiring English sources

The slides were then washed and excess probe was removed by washing in increasingly stringent solutions consisting of 1x SSC (saline sodium citrate) and 0.1x SSC for 10 min at 42°C.

Post-hybridisation, the microarrays were washed in increasingly stringent solutions of SDS and SSC (1 × SSC and 0.1% SDS at 50°C through to 0.1 × SSC at room temperature) and then dried using centrifugation.

GeneChips were washed with a series of non-stringent (25°C) and stringent (50°C) solutions containing variable amounts of MES, Tween20 and SSPE.

GeneChips were washed with a series of non-stringent (25°C) and stringent (50°C) solutions containing variable amounts of MES buffer, tween 20 and SSPE buffer.

Then, the sections were rinsed in 4× SSC (standard saline citrate), treated with RNAse A (20 mg/L) and washed in increasingly stringent SSC solutions at room temperature.

GeneChips were washed with a series of nonstringent (25°C) and stringent (50°C) solutions containing variable amounts of MES, Tween20 and SSPE.

GeneChips were washed with a series of non-stringent (25°C) and stringent (50°C) solutions containing variable amounts of 2-morpholinoethanesulfonic acid, Tween 20, and SSPE (3 M NaCl, 0.2 M, NaH2PO4, 0.02 M EDTA).

Following an overnight incubation at 60°C to hybridize the probe to the membrane, blots were washed twice with wash solution (300 mM sodium chloride, 0.1% sodium dodecyl sulfate, 30 mM sodium citrate) and twice with stringent wash solution (30 mM sodium chloride, 0.1% sodium dodecyl sulfate, 3 mM sodium citrate) for 30 min each at 60°C.

One milliliter of stringent wash solution was then added to each strip and incubated for 15 minutes at 45°C.

After the reaction, 1 mL of warm stringent wash solution (SW) was added to each well, and it was rinsed for 10 20 seconds.

Both the tissue RNA and probe were denatured at 65°C for 10 min. After hybridization, the coverslips were soaked off in Tris-buffered saline with Tween buffer 1X (TBST 10X: 500 nmol/L TrisHCL, pH 7.6, 3 mol/L NaCl, 1% Tween 20), and the tissues were incubated at 55°C for 20 min in stringent wash solution.