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We set stringent settings for base pairing of binding sites (see Materials and Methods) based on this knowledge.
The small inner circles (stringent settings) overlap only marginally.
Cronobacter genes were classified according to the most stringent settings of the trinary output of GACK as present, intermediate or absent.
Analysis of the three sequence regions described above (I homeodomain only, II full-length, III extended homeodomain) was performed using more stringent settings.
The stringent settings used during reference mapping prevented the recruitment of 116 sequences classified as E. coli lambda phage through BLAST.
For the identification of vector sequence, all available DNA databases are queried with less stringent settings.
Similar(36)
The TRACKER program computes pairwise local alignments of all input sequences using BLASTZ [ 42] with non-stringent settings.
The alignments were then treated with the program GBLOCKS with the least-stringent settings to release positions of uncertain homology [ 120].
Significant colocalization of both autophagic proteins with Cx43 was observed (Fig. 7A, panels 1 and 2), even at stringent threshold settings (set to 120 of a maximum intensity of 250 arbitrary units; Fig. 7B, panels 1 and 2).
Searchs were performed under the default settings, except that the Expectation Value (E) was decreased from 10 to the more stringent setting of 1e-5.
The following parameters were changed from default settings: -fastMap, -minIdentity = 99, -maxIntron = 5, and -minScore = 30 (87% of the CENH3 reads and 82% of the control reads could be aligned with the much more stringent setting -minScore = 300).
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