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Besides their efficiency our sequences were devoid of any detectable cytotoxicity, which is probably also due to the stringent selection procedure (Fig. 4).
In a more stringent selection procedure to find genes that are specifically induced by oxygen, only significant 2-fold changes (P < 0·05) were considered.
The bias towards abundant mRNA is probably due to the stringent selection procedure, which filters out genes with weak signals or with missing signals from multiple microarrays.
In general, large banks of a stable protein scaffold are generated by randomization of amino acids in solvent-exposed areas, followed by a stringent selection procedure to retrieve binders from such libraries with desirable characteristics.
We wish to emphasize, however, that for present purposes, a less stringent selection procedure for choosing chromosomal loci with moderate expression capabilities was used to investigate transgene-intrinsic limitations in low-producing antibody variants (exemplified by 2F5scFv-Fc) that might otherwise be overlaid by the high-expression activity of a high-expression locus.
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Despite this large variation, the stringent selection procedures used to extract orthologs make for reliable comparisons as evident by the facts that 1) the ranges of K a values are equivalent across lineages and 2) by removing the lineage with the smallest ortholog set the trend did not change.
As shown in a previous study [27], our study showed that, after having submitted nonagenarian's patients to a stringent selection process, ICU physicians provided similar treatment as to octogenarian patients, namely ventilation procedures, and vasoactive drugs administration, except for the use of RRT for nonagenarians.
A stringent selection process is also vital to maintaining standards, Smith says.
Based on a stringent selection process, 13 putative PDA biomarkers were identified from the public biomarker repository.
The stringent selection process in Germany demands physical and psychological health and fitness.
Thus, our data demonstrate for the first time that a straightforward shRNA-library construction protocol in line with a stringent cellular selection procedure yield potent and readily convertible shRNAs against a viral genome.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com