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Under the relatively stringent sampling conditions (10% and 30%), we observe that attention sampling in normal time is more difficult compared with that during the exogenous events.
A single simulation result cannot be considered representative of a specific mean inlet wind direction – this is evidenced by high variation in the field measurements even after stringent sampling criteria.
Only sampling of males didn't follow this stringent sampling strategy due to the temporal restriction in male availability.
Unfortunately, no CSF samples fulfilling the stringent sampling criteria used in this study were available from patients with mutations in other identified ALS genes.
Thus, if less stringent sampling is applied to small and mediumsized lots, the risk of misclassifying a lot due to sampling uncertainty may be large.
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We chose, however, to analyse the more stringent sample to avoid the influence of parity on the main outcome variables.
Global gene expression is of proven value in interpreting the functional consequence of disease associations (36), but its measurement is expensive and requires stringent sample collection and storage.
Given the strict cutoffs, it is very important that close attention be paid to stringent sample-handling procedures to minimize glycolysis after phlebotomy.
Other intrinsic difficulties include the strong mosaicism recently discovered [ 31- 33], and thus greater sequencing quality and coverage is necessary and more stringent sample selection criteria must be applied.
However, our identified mRNA expression patterns specific for early and advanced stage colorectal tumours are still convincing with our stringent sample selection criteria, high specificity primers and probes as well as reliable statistical analysis.
Third, while Takahashi et al. do not use an analysis strategy that calculates a probability of differential expression, they do use a very stringent sample-wise voting strategy (requiring greater than three-fold differential expression in more than 75% of the patient to normal comparisons) that may in fact be more stringent than the parametric statistical methods we used.
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CEO of Professional Science Editing for Scientists @ prosciediting.com