Sentence examples for stringent read from inspiring English sources

Given the potential for false positives identified in genome sequencing, a stringent read depth of 50 and a call quality of 100 (calculated by complete genomics and based on a phred scale) were chosen as parameters for initial variant filtering.

Using the stringent read mapping criteria described above, read pairs were mapped to all eight alternative conformations.

SNP-o-matic is a fast, memory-efficient and stringent read mapping tool offering a variety of analytical output functions, with an emphasis on genotyping.

We also analyzed breed specificity for 280 putative deletions that resulted from applying a less stringent read mapping quality constraint, which was also applied in previous SV detection studies [ 19, 25].

With a stringent read filter of 30 reads per SNP and twofold AEI ratio, we detect 443 genes with likely AEI in any region such as Huntington's Disease-associated: ELMO1, NTRK2, WNK1 and associated with Schizophrenia: NTRK2, PIK3R1 and RTN4.

We set a stringent read quality for each data set, retaining only those reads with a quality score above 20 in 80% of their nucleotides, and we also restricted the read length to 13 nucleotides (nt) after adapter trimming.

The Obama administration helped prompt the flurry of complaints against colleges in 2011, when it issued a new interpretation of responsibilities under Title IX, and warned that many colleges were even in violation of the previous, less stringent reading of the law.

For characterization of low quality ones, however, VarScan 2 was inferior to the other tools primarily due to its stringent read-depth cutoffs and our application of its high-confidence setting in this study.

This stringent reads-mapping cutoff was used to give high confidence that the genes in this set are not sequencing artifacts or a result of assembly errors, and the 22,315 genes above the 1 CPM cutoff were used for all differential expression and functional analyses.

Nevertheless, it is striking that we obtained these differences despite a stringent minimal read cut-off of 10 reads and 29× (CLC value) medium sequence coverage.

These scaffolds were manually checked with stringent paired read mapping (100% identity) to either confirm the assembly or identify points where chimeras might occur.