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However, chemical synthesis usually involves multiple steps and stringent reaction conditions [3], and resolution methods are limited by theoretical maximum yield of only 50% [4].
Micromixers can also be used to open new operating windows in which controlled polymerization can be performed faster or under less stringent reaction conditions.
In addition, the sol gel catalyst subjected to stringent reaction conditions exhibited an activity enhancement due to the development of some mesoporosity.
Although there are many different types of star polymers and methods for their synthesis, recent advances in the field of controlled radical polymerisation have enabled the facile production of complex star polymer architectures from a large range of monomer families, without the requirement of highly stringent reaction conditions.
More stringent reaction conditions result in complete truncation of carbohydrates from PrPC.
These methods have placed careful emphasis on the amplification step by either rolling circle amplification method [32] or stringent reaction cleanup with reversible magnetic biotin-streptavidin separation [33], followed by design of a PCR run at high annealing temperatures and high cycle numbers, which also could be performed with the use of LNA universal primers [34].
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The stringent response is the initial reaction of microorganisms to nutritional stress.
Nutrient-limited conditions in E. coli trigger a series of reactions known as the stringent response.
gppA encodes an enzyme similar to RelA/SpoT homologs that functions as a mediator of the stringent response, hence coordinating a range of cellular activities in reaction to changes in nutritional abundance [145].
The stringent response is widely observed in bacteria as a stress response in reaction to nutrient starvation [ 23] or various environmental stresses [ 24].
After stringent washes, antibody reactions were revealed using biotinylated anti-species-specific antibodies (BD Biosciences), HRP-conjugated streptavidin (BD Biosciences) and stable DAB (Invitrogen), and using methyl green (Sigma) as counterstain.
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