Approximately half of all detected signals per chromatogram are not reported in the final data set due to these stringent quality control procedures.
Stringent quality control procedures were also employed involving random retyping of 10% samples.
First, the proportion of indeterminate results is higher than in previous studies despite stringent quality control procedures.
Stringent quality control procedures were applied to the SNP data, separately for each platform and breed group.
Earlier studies showed that stringent quality assurance procedures can be used to achieve perfect accuracy, completeness, timeliness and confidentiality [ 20, 22].
Although UFC was analysed at three different central laboratories, they all used the same assays and adhered to stringent quality control procedures.
This should entail adherence to NCI-EORTC recommendations for biomarker validation [ 45], requiring the incorporation of stringent quality control procedures, accurate quantification and scoring methods and unbiased thresholding of expression values.
Experience with current genotyping technologies would suggest that rare variants are more difficult to type than SNPs, and thus stringent quality control procedures are required to avoid increased false-positive error rates as a result of genotype misspecification.
This makes it even more critical that stringent quality control procedures are in place to ensure the veracity of the original information capture, since errors will not only affect the original entry but potentially also be proliferated across multiple closely related records.
Extremely large lots of these two reference RNAs were produced under stringent quality-control procedures.
Following stringent quality control (QC) procedures (see below), the final discovery dataset for analysis consisted of 835 unrelated cases and 5159 controls (plus 717 additional family members, including both parents for 293 of the cases), genotyped at 516 131 autosomal and X-chromosomal SNPs.
