It should be noted that we required very stringent parameters for parsing the groups of orthologous genes.
Feinstein counters that it "puts some very stringent parameters on" the program.
But a meeting with federal investigators -- something Rodriguez is believed not to have had -- comes with more stringent parameters.
The optimal model was validated internally and externally and several statistical stringent parameters were derived.
Mature miRNA sequence homology was one of the stringent parameters considered in our search.
Assembly was conducted with the Celera Assembler using stringent parameters to reduce chimerism and provide a high fidelity consensus sequence.
The use of more or less stringent parameters would result in the identification of fewer or more genes.
Fingerprints of both libraries were assembled using FPC version 8.9 [27] under stringent parameters (cutoff 1e-50 and tolerance 4).
Note that PALS was run with more stringent parameters than BLASTER as it cannot be used with the same values without computing time becoming intractable (see Methods).
One possible explanation is that the inclusion of divergent family members from other species leads to more sparsely connected clusters that need less stringent parameters for correct resolution.
However, as this rule was originally developed for TE copies and not for consensus sequences, we also tested more stringent parameters (Table S9).
