She needed a way of being ample without sacrificing precision; she had to become generously stringent, no easy task.
The rules for patrolling the streets are stringent: no sitting down, no leaning on doors or walls, no cellphone calling, no eating, no smoking, no going into stores.
She's fond of telling a story about being on an Air Jordan flight to the Middle East, when a number of passengers began bristling about the stringent no-smoking-on-Wednesday rule.
Our goal is here to propose a more stringent no-arbitrage condition, which not only accounts for the nonlinearity of the market, but also explicitly refers to a family of contracts under consideration.
To evaluate the accuracy of the L er-0 revised sequence, locus-specific reads were mapped to all three sequences (Col-0, L er-0 draft, and L er-0 revised) using the most stringent parameters (no gaps and no ambiguities were allowed).
For the two least stringent criteria (no cutoff or ≥10 small RNAs mapping to a gene), we observed no significant difference in the microarray expression value for the three categories of protein coding genes (Additional file 1: Figure S5A and Additional file 1: Figure S5B).
On the other hand, adjusting for multiple testing using Bonferroni correction proved to be too stringent, leaving no or few differentially expressed genes.
This can then be adjusted subsequently (i.e., made less stringent) if no variants with a strong candidacy for causality are found after filtering (also see Best Practices section of GATK documentation for variant analysis).
In all four comparisons using the stringent criteria (no deviation from the real methylation values is allowed), MethylExtract yields over 20% more correctly profiled positions compared to Bis-SNP.
After the first round of primer design, with the most stringent conditions (no SNPs in primer annealing region, amplicon length between 250 350 bp, GC content between 30 and 80%%), 97.1 % of all the regions of the 15 genes could be amplified successfully.
Those stringent rules include no talking at meals and no discussion of the crimes (some of them petty, like stealing from a church collection plate) that brought them to Bastoy.
